@article {324136,
	title = {Segregation of molecules at cell division reveals native protein localization.},
	journal = {Nature Methods},
	volume = {9},
	number = {5},
	year = {2012},
	month = {2012 May},
	pages = {480-2},
	abstract = {We introduce a nonintrusive method exploiting single-cell variability after cell division to validate protein localization. We found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers. Retagging five other reportedly foci-forming proteins with the most monomeric fluorescent protein tested suggests that the foci were caused by the fluorescent tags.},
	keywords = {Bacterial Proteins, Cell Division, Endopeptidase Clp, Escherichia coli, Green Fluorescent Proteins, Luminescent Proteins, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Recombinant Fusion Proteins},
	issn = {1548-7105},
	doi = {10.1038/nmeth.1955},
	author = {Landgraf, Dirk and Okumus, Burak and Chien, Peter and Baker, Tania A and Paulsson, Johan}
}
